Distributor Certification Exam

In order to be certified as a distributor of the TrakStation® pH monitoring technology, a passing score of 85% or greater is required on the certification exam. The exam can be taken multiple times until a passing score is achieved.

Once you have passed the certification exam, a certificate on completion will be sent to you for your records. Distributor certification is effective for up to two years from the date of training and exam completion.

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1. Which of the following are true statements about SAFE Sens TrakStation components?

a. The instrument uses drift correction to make small automatic corrections to the pH signal.

b. A sv2 sensor filled with media can be used for a maximum of 7 days

c. The pH system is accurate to ±0.05 pH units

d. All of the above

2. 30-minute continuous pH monitoring can be performed up to:

a. 1 day

b. 7 days

c. 3 days

d. None of the Above

3. True or False: the SAFE Sens system uses the same technology as a pH electrode probe to determine pH

a. True

b. False

4. pH monitoring provides:

a. Immediate knowledge of cell culture environment

b. Potential real-time effects

c. pH trend analysis

d. All of the above

5. A benchtop humidified incubator is using a gas mixture including 8.2% carbon dioxide (CO₂). The lab decides to change the % CO₂ supplied from the previous 8.2% to 6.8% CO_2.What can be expected to happen to the pH of a bicarbonate buffered culture media

a. The pH will increase (become more basic)

b. The pH will decrease (become more acidic)

c. The pH will not change.

6. A bicarbonate buffered culture medium is taken out of the bottle and placed in an sv2 sensor to equilibrate overnight in an incubator supplied with a gas mixture using 5% CO2. The media pH will:

a. Increase (become more basic) until equilibration occurs then remain stable

b. Decrease (become more acidic) until equilibration occurs then remain stable

c. Have no change

7. The following variables can affect the true pH of culture media:

a. Culture media composition

b. Temperature

c. Weather

d. Elevation

e. All of the above

8. When should system alignment be performed using the qc2 tool?

a. Once a day

b. On initial setup and prior to site standardization

c. Once a week

d. Answer a & b

e. Answer b & c

9. Please identify the following pieces of SAFE Sens equipment:

a. A: Trakpod, B: TrakStation, C: sv2 sensor, D. qc2 alignment tool

b. A: Trakpod, B: TrakStation, C: qc2 alignment tool, D. sv2 sensor

c. A: TrakStation, B: Trakpod, C: sv2 sensor, D. qc2 alignment tool

10. Why is site standardization needed?

a. To customize the TrakStation to the physical environment of the lab

b. To immediately change the incubator CO2 settings for a desired media pH

c. To use the lab’s culture media for Trakpod pH calculations and pH reads

d. Answers a & c

e. All of the above

11. What are options for lab staff to access the data collected during pH monitoring?

a. Email data directly from TrakStation (network connected option)

b. Download data using a USB

c. Remote Data Access using the IP address (network connected option)

d. Answers a, b, & c

e. Data is inaccessible

12. Select the answer that accurately orders pH reference samples by best option, with the best option listed first left-to-right.
Example: Best, Second Best, Third Best

Sample 1:
One additional disposable sensor with 150µL of IVF medium to be read using a blood gas analyzer.

Sample 2:
The Certificate of Analysis (CoA) value after duplicating the CO2 settings from the certificate.

Sample 3:
A medium sample created and read similarly to your previously protocol for pH measurement (such as using a pH electrode probe).

a. Sample 1, Sample 2, Sample 3

b. Sample 3, Sample 1, Sample 2

c. Sample 1, Sample 3, Sample 2

13. True or False: Bending, twisting, compressing or pinching the fiber optic cable can irreparably break a Trakpod unit

a. True

b. False

14. Fully-prepared sensors can be saved and held for later use in future pH monitoring

a. True

b. False

15. What are the recommended oil volumes needed to cover media in an sv2 sensor?

a. 50 µL of oil for all incubator types

b. 50 µL of oil for humidified and 100 µL of oil for non-humidified incubators

c. 50 µL of oil for humidified and 150 µL of oil for non-humidified incubators

16. What is the maximum number of Trakpods that can be attached to a TrakStation?

a. 2

b. 5

c. 8

17. True or False: A full 24-hour equilibration time period results in a smoother site standardization and installation

a. True

b. False

18. True or False: The SAFE Sens continuous pH monitoring system requires pH calibration buffers

a. True

b. False

19. How many sensors are recommended to use for site standardization?

a. 2

b. 3

c. 5

20. What cleaning solutions can be used on the sv2 sensors?

a. 70% ethanol solution

b. Mild detergent

c. Peroxide solutions

d. Sensors cannot be cleaned or re-used

21. Which of the below is an acceptable use of sv2 sensors?

a. After 7 days of testing, re-use a sensor for a new test

b. Switching out sensors in the middle of testing

c. Stop testing and then use the same sensor to start new testing

d. None of the above- one sensor per test is best.

Question 1 of 21

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